Journal: Poultry Science

Article Title: New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

doi: 10.1016/j.psj.2024.103674

Figure Lengend Snippet: The primers involved in this study.

Article Snippet: GPX4 , 1:500 , Boster Biotechnology, China , A02059-1.

Techniques:

Journal: Poultry Science

Article Title: New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

doi: 10.1016/j.psj.2024.103674

Figure Lengend Snippet: The antibodies used in this study.

Article Snippet: GPX4 , 1:500 , Boster Biotechnology, China , A02059-1.

Techniques:

Journal: Poultry Science

Article Title: New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

doi: 10.1016/j.psj.2024.103674

Figure Lengend Snippet: MPs induced ferroptosis in spleen. (A) Western Blot results in ferroptosis-related proteins. (B) Quantitative analysis of GPX4, FTH1, and SLC7A11 protein expression. (C) Ferroptosis-related factors of mRNA expression changes. Data were expressed as mean ± SD, n = 3. *, **, ***, denotes: p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: GPX4 , 1:500 , Boster Biotechnology, China , A02059-1.

Techniques: Western Blot, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Complement 5a‐mediated trophoblasts dysfunction is involved in the development of pre‐eclampsia

doi: 10.1111/jcmm.13466

Figure Lengend Snippet: Expression of angiogenesis‐related factors in human placenta. ( A ) Relative mRNA levels of anti‐angiogenic factors ( IL ‐1β, TNF ‐α, IL ‐6, MCP ‐1 and sF lt1) and pro‐angiogenic ( PIGF , IL ‐10) in normal and pre‐eclamptic placentas. Data are represented as mean ± S.E.M. N = 4–6 in each group. * P < 0.05, ** P < 0.01, *** P < 0.001. ( B ) Immunofluorescence analysis of representative angiogenesis‐related factors (red) and Cy7 (green) in normal and PE placentas. Nuclei were counterstained with DAPI (blue). Scale bar: 60 μm.

Article Snippet: The human placenta slices were blocked in 10% normal goat serum for 30 min., then incubated with primary antibodies against CD31 (Santa Cruz), CD11b (BD Biosciences, San Jose, CA, USA), C5a (Comp Tech, A221), C5aR (Biolegend), cytokeratin 7 (Cy7, Santa Cruz), PIGF (Proteintech group, Wuhan, Hubei, China), sFlt1 (Life Technologies, Waltham, MA, USA), IL‐1β (Boster), IL‐6 (Boster) and MCP‐1 (Boster, Wuhan, Hubei, China) at 4°C overnight.

Techniques: Expressing, Immunofluorescence

Journal: Journal of Cellular and Molecular Medicine

Article Title: Complement 5a‐mediated trophoblasts dysfunction is involved in the development of pre‐eclampsia

doi: 10.1111/jcmm.13466

Figure Lengend Snippet: Trophoblasts stimulated with C5a display an anti‐angiogenic phenotype. ( A ) HTR ‐8/ SV neo cells treated with C5a showed a polarization towards an anti‐angiogenic phenotype with significantly increased mRNA levels of IL ‐1β, TNF ‐α, IL ‐6, MCP ‐1, sF lt1 and decreased mRNA level of PIGF and IL ‐10. The respective mRNA was normalized to β‐actin housekeeping gene. Data are represented as mean ± S.E.M . N = 3 in each group * P < 0.05, ** P < 0.01. ( B ) Immunofluorescence staining for sF lt1 and PIGF expression in HTR ‐8/ SV neo cells in the presence of C5a or PBS ( CON ). Scale bar: 60 μm.

Article Snippet: The human placenta slices were blocked in 10% normal goat serum for 30 min., then incubated with primary antibodies against CD31 (Santa Cruz), CD11b (BD Biosciences, San Jose, CA, USA), C5a (Comp Tech, A221), C5aR (Biolegend), cytokeratin 7 (Cy7, Santa Cruz), PIGF (Proteintech group, Wuhan, Hubei, China), sFlt1 (Life Technologies, Waltham, MA, USA), IL‐1β (Boster), IL‐6 (Boster) and MCP‐1 (Boster, Wuhan, Hubei, China) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Expressing

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 8. Effects of TFE from Folium isatidis on the genes expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d) and p65 (e) in lungs tissues of LPS induced ALI mice ( # compared with the control, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing, Control

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 9. Effects of TFE from Folium isatidis on the proteins expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d), p-I 𝜅B (e), p65 (f) and p-p65 (g) in lungs tissues of LPS induced ALI mice ( # compared with the cntrol, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 10. Regulation of TFE from Folium isatidis on TLR4-TRIF-NF- 𝜅B signaling pathway in LPS induced ALI mice.

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: